Help whats wrong: noise / guiding error?

That is weird yes, could you post some screenshots of a single sub of your first session and one from your second? As well as a screenshot of you masterdark, masterflat etc?

Also check in the header of the images in your first and second session, if you didn't change any options by accident

@vincent-mod 

First session (2hr)  typical sub is below

Second session (6.5 hr) typical sub is below

First session master flat

First session master dark

First session master bias is below

Second session master flat is below

Second session master dark is below

Second session master bias is below

Thanks!

It looks a bit like your flat isn't exposed properly and there might be a bit of light leakage in your dark. Normally you want to see a histogram that has a curve that drops quite sharply, but I see an extra bump in yours. I can have a look at your data here if you want as well, but I think there are a few issues in there. What happens if you don't use any calibration frames?

@vincent-mod 

thanks. Redoing without the calibration frames made it better. This is the APP result

With some minor denoising, I was able to get this

So I am wondering what went wrong with my calibration frames. I am using a Optolong Lpro filter. So what should I expect the different calibration subs / masters look like? 

Like I mentioned, you probably want to look at the darks/bias and remake them in a totally dark environment. Like in a bag in a closet or something. Do make them with the same settings as the lights, including temperature if possible. That, in combination with a slightly longer exposed flat, may work better. If not, I can have a look at the new data.